https://submissions2.mirasmart.com/Verify/CEPharm2019/submission/temp/rad3B853.pdf, Du Y, Walsh A, Ehrick R et al (2012) Chromatographic analysis of the acidic and basic species of recombinant monoclonal antibodies. PubMed View all citations for the Imaged Capillary Electrophoresis (iCE) platform. The product range includes Maurice S, Maurice C, iCE3. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE Technical University of Braunschweig in Germany, "The short run times and easy sample preparation allowed for method development within a day or two. ", Joan Garrison, PubMed A broad range of protein therapeutics such as monoclonal antibodies, antibody-drug conjugates (ADCs), and fusion proteins are routinely analyzed by icIEF due to its high resolution and high reproducibility. These tools are designed to resolve problems related to protein analysis and reveal insights into the nature of proteins. This technical note introduces SimpleSol, a novel and alternative reagent to urea to solubilize proteins for icIEF analysis on iCE and Maurice systems. ProteinSimple is known for manufacturing and supplying analytical instrumentation and consumables for protein research. Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments. As they are known to intercellular communicators, they take part in numerous cellular and disease processes. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool, SimpleSol - a Novel Protein Solubilizer for Capillary Isoelectric Focusing Analysis Technical Note. View all citations for the Imaged Capillary Electrophoresis (iCE) platform. ProteinSimples portfolio of protein tools includes a wide range of products, from simple western immunoassay systems that quantify protein expression to biological systems that probe the structure and purity of protein-based therapeutics. In this article, we will review ProteinSimples product portfolio and applications of its instruments in various domains of medical research. These include: (1) nucleoproteins (2) proteoglycans and glycoproteins (3) chromoproteins (4) phosphoproteins (5) lipoproteins and (6) metalloproteins (Murray et al., 2006; Watford & Wu, 2018 ). ACD branded products will be available on biotechne.com in the near future. See terms and conditions for offer details. Correspondence to These contain simple protein combined with nonprotein prosthetic group (Murray et al., 2006). Am Pharm Rev 14:8791, Loughney JW, Ha S, Rustandi RR (2017) Quantitation of CRM197 using imaged capillary isoelectric focusing with fluorescence detection and capillary Western. They even have an interactive Simple Western Kit Builder to help in your assay planning. USA, TEL 612 379 2956 Simple Western Systems come with gel-free, blot-free and hands-free technology to help you obtain perfect data. This is a preview of subscription content, access via your institution. As the name suggests, the company aims to simplify protein research and help thousands of researchers around the world with their diverse portfolio of protein tools. Minneapolis, MN 55413 As the name suggests, the company aims to simplify . Simple Western Systems help perform serum antibody analysis, and multi-analyte characterization of Humoral response. Fisher Scientific is always working to improve our content for you. ProteinSimple also conducts webinars and training sessions to help consumers learn more about their products. By continuing to use this website you acknowledge this and agree to our cookie policy. FAX 612 656 4400. CE-SDS & icIEF iCE Platform |ProteinSimple - Bio-Techne Imaged capillary isoelectric focusing (icIEF) is a gold standard method for characterizing the charge heterogeneity of protein therapeutics. With a comprehensive range of analytical instruments, ProteinSimple provides its customers an easier, faster, and more affordable way to conduct protein analysis. Catalog No. These are easy, high-performing and budget-friendly. By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. Protein analysis tools arent the only thing we lease, though. https://doi.org/10.1080/19420862.2019.1628589, CrossRef Headquartered in Orchard Parkway, San Jose, California, ProteinSimple is helping scientists discover answers to real-world problems through protein and disease research. Presented at CE in the Biotechnology & Pharmaceutical Industries: symposium on the practical applications for the analysis of proteins, nucleotides and small molecules, Monday, Sept 30, 2019, Bethesda, MD, USA. Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. For safe and effective treatments using cell and gene therapies, ProteinSimple has a range of next-generation analytical solutions to offer. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. J Chromatogr B Biomed Appl 714:113118. Using TcBusterTM Technology you can carry out genome engineering with non-viral transgene insertion. Maurice produces pI and charge heterogeneity data in less than 10 minutes, and size-based CE-SDS data in 35 minutes. In regard to the volume of applicable uses of their products, they are immense. Learn more, Hjertn S, de Zhu M (1985) Adaptation of the equipment for high-performance electrophoresis to isoelectric focusing. SimpleSol Protein Solubilizer, 200 mL. Not finding your part number? Technical Data. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE Their tools have facilitated a high-performance workflow of EV protein characterization. ProteinSimple recently merged with Bio-Techne, and is now a part of the companys Protein Platforms division. SimpleSol Protein Solubilizer for cIEF (046-575) | Bio-Techne In a single sequencing run, it can measure total protein expression in thousands of cells. Preparation & Storage. We also lease a wide variety of lab equipment, from general lab, clinical, and analytical instruments to spectroscopy and biotech equipment. Eastern North America. 1w. CAS Browse your leasing options today! ProteinSimple SimpleSol Protein Solubilizer for cIEF, 200mL - Fisher Sci This helps get precise, quantitative data for accurate determination of DC50 curve values. MFI Bot1 Autosampler is also available, and can be added anytime to automate the whole process. ProteinSimple. Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing. (eds) Proteomic Profiling. To uncover the role of proteins in cancer and accelerate the analysis of protein-based therapeutics, ProteinSimples Advanced Western Blotting solutions make the study of proteins, their function, and signaling mechanisms easier. We will not share your information for any other purposes. Bio-Techne uses cookies to provide you with a great website experience. Bio-Techne is known for developing, manufacturing, and selling biotechnology and reagents for protein analysis and, through this merger, will now offer ProteinSimples analytical instruments as well. MFI is based on micro-flow imaging that helps you know more about particles in your sample and aids in rapid and robust sample analysis. And because proteins tend to aggregate less at lower concentrations you can reduce or even eliminate urea completely in some of your methods. The Single Cell Western Technology on Milo allows the user to profile heterogeneity in complex samples, simplify phospho-flow signaling studies, measure protein isoform homogeneity, and more. With fully automated Simple Westerns you can quantify Degrader induced protein knockdown even with low sample volume. ProteinSimple is known for manufacturing and supplying analytical instrumentation and consumables for protein research. Methods in Molecular Biology, vol 2261. FAX 612 656 4400, 15% Off Limited-time Savings on Your Reagents, Assays and Kits. Part of Springer Nature. Supplier: ProteinSimple 046575. Bio-Techne uses cookies to provide you with a great website experience. Compass for iCE, the software package Maurice uses to acquire, manage and analyze data, has all the tools required for 21 CFR compliance so you'll have no issue analyzing biotherapeutics on Maurice in a regulated environment. Electrophoresis, Western Blotting and ELISA, Chromatography and Mass Spectrometry Reagents, Laboratory Syringe Needles and Accessories, Lab Coats, Aprons, and Other Safety Apparel, Sharps Disposal Containers and Accessories, Classroom Laboratory Supplies and Consumables, Applied Biosystems TaqMan Assay and Arrays Search Tool, Applied Biosystems TaqMan Custom Assay Design Tools, Applied Biosystems Custom qPCR Primers and TaqMan Probes Tool, Chemical Storage and Management Resource Center. https://doi.org/10.1002/elps.201800118, Zhang X, Voronov S, Mussa N, Li Z (2017) A novel reagent significantly improved assay robustness in imaged capillary isoelectric focusing. PubMed Central Ella automates every step of the immunoassay workflow. Christopher Heger . Their application is seen in PCR tests for SarsCoV2, Exosomes, Proteomic research, Targeted Protein Degradation and Cancer research. SimpleSol Protein Solubilizer, 200 mL. Using these systems, heterogeneity in Cancer and Immuno-oncology samples can be studied. ProteinSimple branded instruments are available to quote. The product is shipped with polar packs. ProteinSimple offers the worlds first automated Single-Cell Western platform: MiloTM. Toll-free 800 343 7475 In all tests, more than three injections were performed from a single sample preparation under each condition to observe the reproducibility. ProteinSimple branded instruments are available to quote. Your feedback has been submitted. Platform methods, which can be applied without modification to the analysis of different protein therapeutics, save valuable time and resources in method development and quality control. Whether it is vaccine development workflow, bioprocessing, gene therapy or any other life science process, these products and consumables have a lot to offer. ProteinSimples Simple WesternTM Platform is a fully automated system that provides data on 24 samples in only 3 hours. Western Protein Analysis problem solvers include Jess, Abby, Wes, Sally Sue, Peggy Sue, and NanoPro 1000. ", Professor Hermann Wtzig, Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. Chris Heger, Ph.D. - Director, Applications Science - LinkedIn Google Scholar, Salas-Solano O, Kennel B, Park SS et al (2012) Robustness of iCIEF methodology for the analysis of monoclonal antibodies: an interlaboratory study. Using dTag degradation technology, you can target and degrade any intracellular protein of interest. https://www.proteinsimple.com/literature_download.html?docid=1817, ProteinSimple, a Bio-Techne Brand, San Jose, CA, USA, Jiaqi Wu,Will McElroy,Charles Haitjema,Carsten Lck&Christopher Heger, You can also search for this author in All contact information provided shall also be maintained in accordance with our ProteinSimples products and solutions will soon be available on Bio-Technes website to enable consumers to access the brands products and services in one place. ProteinSimple. Toll-free 800 343 7475 J Chromatogr A 346:265270. You can develop platform methods and use them for multiple molecules too. "I think that the data we produced [on Maurice] shows excellent quality, due to the precise and reliable Maurice instrument. Which Brands are Currently Available on biotechne.com? Maurice's native fluorescence detection for cIEF provides 3-5X higher sensitivity than UV absorption, which means you can now measure sample at concentration as low as 0.7 g/ml. See more. Jan 2015 - Oct 20161 year 10 months. Store the unopened product at 2 - 8 C. Ella . For use with Maurice, Maurice C. and iCE3 systems. Exosomes and Extracellular vesicles (EV) are a potential source of biomarker discovery and for liquid biopsy analysis. It allows improved reproducibility, quantitation, throughput, and time to market thereby speeding up the vaccine development workflow. Please use the form below to provide feedback related to the content on this product. Google Scholar, Wu G, Yu C, Wang W, Wang L (2018) Interlaboratory method validation of icIEF methodology for analysis of monoclonal antibodies. SimpleSol Protein Solubilizer for cIEF (046-575). MAbs 4:578585. SimpleSol - a Novel Protein Solubilizer for Capillary Isoelectric Manager, Field Applications. Their products are being used in fields like neuroscience, oncology, bioinformatics, RNA research, cell and gene therapy, and more. A platform method for charge heterogeneity - ScienceDirect To access this literature content please fill out the form below. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. Copyright 2023 Bio-Techne. In addition, when dealing with molecules such as ADCs, no more hassle about handling the toxic waste. Google Scholar, Liu Z, Pawliszyn J (2005) Behaviors of the MS2 virus and related antibodies in capillary isoelectric focusing with whole-column imaging detection.
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