Get resources and offers direct to your inbox. No. Carcinogenesis associated with human papillomavirus infection. Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. waste container. Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party Xu X,Pang J,Chen Y,Bucala R,Zhang Y,Ren J. 130-130-708 30 tests in 60 L One test corresponds to labeling of 106 cells in a final volume of 100 L (applies only to flow cytometry). American journal of physiology. Xia MY,Zhao XY,Huang QL,Sun HY,Sun C,Yuan J,He C,Sun Y,Huang X,Kong W,Kong WJ. Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms Cyclins and cyclin-dependent kinases (cdks) form active complexes that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. Song FB, Du H, Xiao AM, Wang C, Huang X, Yan PS, Liu ZH, Qu XF, Belinson JEROMEL, Wu RF. Bergeron C, Ronco G, Reuschenbach M, Wentzensen N, Arbyn M, Stoler M, von Knebel Doeberitz M. Int J Cancer. All reagents required for this protocol may be efficiently purchased together in our Intracellular Flow P16INK4A FCM was built upon a novel antibody clone and a series of positive and negative (p16INK4A -knockout) standards. Permeabilize cells by adding ice-cold 100% methanol slowly to pre-chilled cells, while gently vortexing, to a final concentration of 90% methanol. Since 2018, 24 100-women (HPV-positive/-negative, Pap-normal/-abnormal) have been enrolled nationwide for two-tier validation work. Do you want to continue? Stojiljkovic MR,Ain Q,Bondeva T,Heller R,Schmeer C,Witte OW. Have you cited this product in a publication? During the process, a sample of cells or particles is suspended in fluid and injected into a flow cytometer machine. The site is secure. including for use or application of the Thermo Fisher Scientific products presented. This form is intended to help us improve our website experience. UniProt ID: Wash cells by centrifugation in excess 1X PBS to remove methanol. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics. 2019;84(7):782799. Discard supernatant in appropriate Would you like email updates of new search results? A cell cycle regulator potentially involved in genesis of many tumor types. LncRNA ABHD11-AS1 promotes the development of endometrial carcinoma by targeting cyclin D1. 550946) together with the DAB Substrate Kit (Cat. critical factor. For Research Use Only. (Biology). No. The biochemical properties of p16 suggest that it may be a tumor suppressor gene product. Counteracting survival functions of EBNA3C in Epstein-Barr virus (EBV)-driven lymphoproliferative diseases by combination of SAHA and bortezomib. Application Key: WB -Western Blot IP -Immunoprecipitation IHC -Immunohistochemistry ChIP -Chromatin Immunoprecipitation C&R -CUT&RUN C&T -CUT&Tag DB -Dot Blot eCLIP -eCLIP IF -Immunofluorescence F -Flow Cytometry Species Cross-Reactivity Key: CST Product Terms of Sale and any applicable Discard supernatant in appropriate waste container. Huang W,Zhong W,Xu J,Su B,Huang G,Du J,Liu Q. Patient-derived bladder cancer xenografts in the preclinical development of novel targeted therapies. For other support, Wang H,Zhong Q,Yang T,Qi Y,Fu M,Yang X,Qiao L,Ling Q,Liu S,Zhao Y. Purified Mouse Anti-Human p16 - BD Biosciences 1995; 55(3):493-497. Tam SW, Shay JW, Pagano M. Differential expression and cell cycle regulation of the cyclin-dependent kinase 4 inhibitor p16Ink4. representative of CST, are rejected and are of no force or effect. The p16INK4A -positive ratio was 13.9 1.8% among HPV-negative NILM women and peaked at the ages of 40-49 years; after HPV infection, the ratio increased to 15.1 1.6%, varying with the carcinogenesis of the viral genotype. 2015 Jun 15;136(12):2741-51. doi: 10.1002/ijc.28900. Feng T,Shao F,Wu Q,Zhang X,Xu D,Qian K,Xie Y,Wang S,Xu N,Wang Y,Qi C. N-Stearoyl-L-Tyrosine Inhibits the Senescence of Neural Stem/Progenitor Cells Induced by A 1-42 via the CB2 Receptor. Clipboard, Search History, and several other advanced features are temporarily unavailable. P42771. This antibody does not cross-react with p15 INK4B. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Not for Use in Diagnostic Procedures. UniProt ID: IHC-P: Human endometrial cancer and esophageal cancer tissue. copyright notices or markings, (d) use the Products solely in accordance with In cohort studies, the 2-year prognostic values of p16INK4A were investigated with other risk factors by multivariate regression analyses in three cervicopathological conditions: HPV-positive Pap-normal, Pap-abnormal biopsy-negative and biopsy-confirmed LSIL. The p16 protein has been identified as a specific inhibitor of cdk4 because it blocks cdk4 substrate phosphorylation. BD Pharmingen FITC Mouse Anti-Human p16 Set. 550524) is required. Any use of Product for diagnostic, By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. STRING - Known and Predicted Protein-Protein Interactions. You are now leaving the BD Biosciences website. This gene generates several transcript variants which differ in their first exons. (Biology). ), please refer to the dye product page for the recommended protocol. ), please refer to the dye product page for the recommended protocol. Get resources and offers direct to your inbox. Conduct a small-scale experiment if you are unsure. Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. P16 Monoclonal Antibody (OTI4C11) (CF500036) - Thermo Fisher Scientific p21 Waf1/Cip1 (12D1) Rabbit mAb (PE Conjugate) #8865 The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. and 1:500 Anti-CDKN2A/p16 INK4a (EPR1473, Abcam, ab209579) antibody was added and incubated at room temperature for 30 min in the dark. Visit www.cellsignal.com for a full listing of cellular dyes validated for use in flow cytometry. All other trademarks are the property of their respective owners. Motterle A,Pu X,Wood H,Xiao Q,Gor S,Ng FL,Chan K,Cross F,Shohreh B,Poston RN,Tucker AT,Caulfield MJ,Ye S, Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved. Damsky W,Micevic G,Meeth K,Muthusamy V,Curley DP,Santhanakrishnan M,Erdelyi I,Platt JT,Huang L,Theodosakis N,Zaidi MR,Tighe S,Davies MA,Dankort D,McMahon M,Merlino G,Bardeesy N,Bosenberg M. Ocular expression and distribution of products of the POAG-associated chromosome 9p21 gene region. Flow Cyt: HeLa cells. Keywords: Cancer Res. Flow cytometric analysis of p16 expression on WI-26 VA4 human lung cells. Recently a gene cloned from the short arm of human chromosome 9, Multiple Tumor Suppressor 1 (MTS1) has been identified as the gene for p16]. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc. Resuspend cells in 100 l of diluted primary antibody, prepared in Antibody Dilution Buffer at a recommended dilution or as determined via titration. Mix well to dissociate pellet and prevent cross-linking of individual cells. Compare Anti-p16INK4a Flow Cytometry Antibody Products from leading suppliers on Biocompare. Flow cytometry. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. Mix well to dissociate pellet and prevent cross-linking of individual cells. These include superior lot-to-lot consistency, continuous supply, and animal-free manufacturing. Products sold or licensed by CST from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from Pellet cells by centrifugation and remove supernatant. American journal of physiology. Analyte Specific Reagent. Not for use in diagnostic or therapeutic procedures. NOTE: When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc. 550946) together with the DAB Substrate Kit (Cat. Properties biological source mouse Quality Level 200 recombinant expressed in HEK 293 cells conjugate unconjugated antibody form purified antibody antibody product type primary antibodies clone BD FACSLyric Flow Cytometer Integrated with the BD FACSDuet Sample Preparation System, BD Rhapsody Express Single-Cell Analysis System, BD Rhapsody TCR/BCR Profiling Assays for Human and Mouse, BD Rhapsody Whole Transcriptome Analysis (WTA) Amplification Kit, Industry Solutions (Biotech, Pharma, CRO), Bulk Reagents, Custom Products and Solutions, View All Industry Solutions (Biotech, Pharma, CRO), p16-INK4, p16-INK4a, ARF, MTS1, CDKN2, CDK4l, Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development). Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala143 of human p16 INK4A protein. (Clone-specific). Li Y, Nichols MA, Shay JW, Xiong Y. Transcriptional repression of the D-type cyclin-dependent kinase inhibitor p16 by the retinoblastoma susceptibility gene product pRb. NOTE: Adherent cells or tissue should be dissociated and in single-cell suspension prior to fixation. The present study was designed to evaluate whether the combined use of Syto16, the Pgp blocker PSC833, and 7-AAD allows simultaneous detection of all parameters, with emphasis on applications in acute myeloid leukemia (AML). 550878). For paraffin sections, microwave oven pretreatment with BD Retrievagen A (pH 6.0) (Cat. antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. Link to hereditary melanoma brightens mood for p16 gene. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. No. Anti-P16 Flow Cytometry Antibody Products Products (75) User Reviews (1) Company View Product View Your search returned 75 P16 Flow Cytometry Antibodies across 13 suppliers. Anti-P16 Antibody Products | Biocompare Agonists, activators, antagonists and inhibitors, HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080), See all CDKN2A/p16INK4a primary antibodies, Goat Anti-Mouse IgG H&L (Alexa Fluor 488) (, Goat Anti-Mouse IgG H&L (DyLight 488) preadsorbed (, PE / R-Phycoerythrin Conjugation Kit- Lightning-Link (, Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (, Recombinant Human CDKN2A/p16INK4a protein (. Epub 2014 May 12. While polyclonal antibodies are After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line Is p16(INK4A) expression more useful than human papillomavirus test to determine the outcome of atypical squamous cells of undetermined significance-categorized Pap smear? Product Details Product Specific Information 10883-1-AP reacts with p16 specifically. Antibodies for Flow Cytometry | Thermo Fisher Scientific - US Since applications vary, each investigator should titrate the reagent to obtain optimal results. 2005 Apr;97(1):35-40. doi: 10.1016/j.ygyno.2004.11.034. 550880). The p16 protein inhibits the activity of cyclin-dependent protein kinases (CDK4 and CDK6) to prevent the phosphorylation of the retinoblastoma protein (Rb) and subsequently lead to the accumulation of the hypophosphorylated form of Rb that mediates cell-cycle arrest 1.Studies have shown that abnormal expression of p16 is frequently associated with different types of human tumors, such as . As p16 INK4a mRNA is readily detected in murine or human peripheral blood T cells and increases with aging . Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Discard supernatant in appropriate Moreover, since the recombinant antibody sequence is known, continuity of supply This antibody is expected to exhibit the same species cross-reactivity as the unconjugated p16 INK4A (D7C1M) Rabbit mAb #80772. requires a separate license from CST. HHS Vulnerability Disclosure, Help Aromseree S, Wongjumpa W, Ekalaksananan T, Temtanakitpaisan A, Kleebkaow P, Srisathaporn S, Tongchai P, Pientong C. Asian Pac J Cancer Prev. Molecular pathways: cDK4 inhibitors for cancer therapy. 2023 BD. 1994; 264(5157):436-440. 550337) as the secondary antibody and Streptavidin-HRP (Cat. Journal of the American Heart Association, Altieri P,Barisione C,Lazzarini E,Garuti A,Bezante GP,Canepa M,Spallarossa P,Tocchetti CG,Bollini S,Brunelli C,Ameri P. Evaluation of Protein Profiles From Treated Xenograft Tumor Models Identifies an Antibody Panel for Formalin-fixed and Paraffin-embedded (FFPE) Tissue Analysis by Reverse Phase Protein Arrays (RPPA). Winkler C,Rouget R,Wu D,Beullens M,Van Eynde A,Bollen M. Cell-nonautonomous local and systemic responses to cell arrest enable long-bone catch-up growth in developing mice. by the FDA or other regulatory foreign or domestic entity, for any purpose. documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or (Biology). Therapeutic Challenge with a CDK 4/6 Inhibitor Induces an RB-Dependent SMAC-Mediated Apoptotic Response in Non-Small Cell Lung Cancer. Repeat if necessary. The biochemical properties of p16 suggest that it may be a tumor suppressor gene product. Aformalin-fixed, paraffin-embedded section from human breast tissue was stained with the Mouse Anti-Human p16 antibody (Cat. Lentivirus-mediated gene silencing of NOB1 suppresses non-small cell lung cancer cell proliferation. Looks like you're visiting us from {{countryName}}. Cyclins and cyclin-dependent kinases (cdks) form active complexes that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. PMC Store at 4C. Science. CDKN2A/p16 Antibody (JC8) | SCBT - Santa Cruz Biotechnology Jger W,Xue H,Hayashi T,Janssen C,Awrey S,Wyatt AW,Anderson S,Moskalev I,Haegert A,Alshalalfa M,Erho N,Davicioni E,Fazli L,Li E,Collins C,Wang Y,Black PC.